Renal adenylate cyclase activation by amino acylated vasopressin and oxytocin

Two series of neurohypophysial peptide amino-acylated derivatives were tested for their ability to activate plasma membrane adenylate cyclase prepared from pig or rat kidney. They were firstly [8-lysine]-vasopressin-related derivatives (N a[Glycyl-Cys] 1-[8-Lysine]-vasopressin and N a-[Glycyl-Glycyl...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Molecular and cellular endocrinology 1975, Vol.2 (2), p.69-79
Hauptverfasser: Barth, Tomislav, Roy, Christian, Rajerison, Rabary, Jard, Serge
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Two series of neurohypophysial peptide amino-acylated derivatives were tested for their ability to activate plasma membrane adenylate cyclase prepared from pig or rat kidney. They were firstly [8-lysine]-vasopressin-related derivatives (N a[Glycyl-Cys] 1-[8-Lysine]-vasopressin and N a-[Glycyl-Glycyl-Glycyl-Cys] 1-[8-Lysine]-vasopressin) and secondly oxytocin-related derivatives (N a-[Glycyl-Cys] 1-oxytocin, N a-[Leucyl-Glycyl-Glycyl-Cys] 1-oxytocin, and N a-[Glycyl-Cys] 1-[2-O-methyl tyrosine]-oxytocin). The maximal adenylate cyclase activation induced by these peptides was lower than that induced by their respective parent hormones. After incubation of these analogues with plasma membranes obtained from the renal medulla, no significant release of parent hormones occurred. Good qualitative correlations were observed between relative antidiuretic activities measured in vivo and relative potencies in activating adenylate cyclase. It was concluded that direct action of the peptides tested on the kidney is at least partly responsible for their antidiuretic activity in vivo.
ISSN:0303-7207
1872-8057
DOI:10.1016/0303-7207(75)90049-0