Selection of optimal set of wavelengths for analysis of hyperchromic spectra
The formulae of mean-square deviations of fractions of denatured base pairs in dependence of temperature have been used for selection of optimal set of wave-lengths suited for the study of thermal denaturation dispersion of DNA and DNA complexes. A method is described, which enables the study of the...
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| Veröffentlicht in: | Analytical biochemistry 1975-06, Vol.66 (2), p.545-555 |
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| container_end_page | 555 |
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| container_issue | 2 |
| container_start_page | 545 |
| container_title | Analytical biochemistry |
| container_volume | 66 |
| creator | Raukas, Ergo Kooli, Kai |
| description | The formulae of mean-square deviations of fractions of denatured base pairs in dependence of temperature have been used for selection of optimal set of wave-lengths suited for the study of thermal denaturation dispersion of DNA and DNA complexes. A method is described, which enables the study of the first stage of thermal denaturation of complexes of DNA with basic polypeptides in terms of A · T and G · C base pairs. |
| doi_str_mv | 10.1016/0003-2697(75)90622-3 |
| format | Article |
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A method is described, which enables the study of the first stage of thermal denaturation of complexes of DNA with basic polypeptides in terms of A · T and G · C base pairs.</description><subject>Binding Sites</subject><subject>DNA</subject><subject>Mathematics</subject><subject>Methods</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic Acid Denaturation</subject><subject>Peptides</subject><subject>Protein Binding</subject><subject>Spectrophotometry, Ultraviolet - methods</subject><subject>Temperature</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo67r6DxR6Ej1UM0k_L4IsfsGCB_Uc0nTiRrpNTbor--9N7aI3T8PwzvvOzEPIKdAroJBdU0p5zLIyv8jTy5JmjMV8j0yBlllMOS33yfR35JAcef9BKUCSZhMyAeA5AEzJ4gUbVL2xbWR1ZLverGQTeeyH9ktugtq-90sfaesi2cpm640ftOW2Q6eWzq6MinwXMpw8JgdaNh5PdnVG3u7vXueP8eL54Wl-u4gVT_M-ThUir3RNMwgHIi8yppEVEjQipBWrIJzMSpBFWSSosEx4hbqodYG5rhH4jJyPuZ2zn2v0vVgZr7BpZIt27UURzCwPthlJxkHlrPcOtehceNBtBVAxQBQDITEQEnkqfiAKHmxnu_x1tcL6zzRSC_rNqGN4cmPQCa8Mtgpr4wIIUVvz_4JvzHaBqw</recordid><startdate>197506</startdate><enddate>197506</enddate><creator>Raukas, Ergo</creator><creator>Kooli, Kai</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197506</creationdate><title>Selection of optimal set of wavelengths for analysis of hyperchromic spectra</title><author>Raukas, Ergo ; Kooli, Kai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-5cee3bfd061000e3862fe28a1fee15b2b1269291a8984ece943bef8df8e7fde13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Binding Sites</topic><topic>DNA</topic><topic>Mathematics</topic><topic>Methods</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic Acid Denaturation</topic><topic>Peptides</topic><topic>Protein Binding</topic><topic>Spectrophotometry, Ultraviolet - methods</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raukas, Ergo</creatorcontrib><creatorcontrib>Kooli, Kai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raukas, Ergo</au><au>Kooli, Kai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selection of optimal set of wavelengths for analysis of hyperchromic spectra</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1975-06</date><risdate>1975</risdate><volume>66</volume><issue>2</issue><spage>545</spage><epage>555</epage><pages>545-555</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>The formulae of mean-square deviations of fractions of denatured base pairs in dependence of temperature have been used for selection of optimal set of wave-lengths suited for the study of thermal denaturation dispersion of DNA and DNA complexes. 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| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1975-06, Vol.66 (2), p.545-555 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82912794 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Binding Sites DNA Mathematics Methods Nucleic Acid Conformation Nucleic Acid Denaturation Peptides Protein Binding Spectrophotometry, Ultraviolet - methods Temperature |
| title | Selection of optimal set of wavelengths for analysis of hyperchromic spectra |
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