Selection of optimal set of wavelengths for analysis of hyperchromic spectra

The formulae of mean-square deviations of fractions of denatured base pairs in dependence of temperature have been used for selection of optimal set of wave-lengths suited for the study of thermal denaturation dispersion of DNA and DNA complexes. A method is described, which enables the study of the...

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Veröffentlicht in:	Analytical biochemistry 1975-06, Vol.66 (2), p.545-555
Hauptverfasser:	Raukas, Ergo, Kooli, Kai
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites DNA Mathematics Methods Nucleic Acid Conformation Nucleic Acid Denaturation Peptides Protein Binding Spectrophotometry, Ultraviolet - methods Temperature
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	The formulae of mean-square deviations of fractions of denatured base pairs in dependence of temperature have been used for selection of optimal set of wave-lengths suited for the study of thermal denaturation dispersion of DNA and DNA complexes. A method is described, which enables the study of the first stage of thermal denaturation of complexes of DNA with basic polypeptides in terms of A · T and G · C base pairs.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(75)90622-3