Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique

A rapid, simple, sensitive and reproducible filter-paper assay was developed to follow the glucose transfer from uridine diphosphate [ 14C]glucose to protein or protein-bound oligosaccharides. This method could be useful for following the formation of any glycoprotein. Rectangles of trichloroacetic acid-treated filter paper are introduced into a test tube containing the reaction mixture. The papers are submitted to a batch washing-procedure that ensures the removal of the compounds that are not trichloroacetic acid-precipitable. After scintillation counting, the paper can be reused to study the acid lability of the glucoprotein.