A simple technique for eliminating interference by detergents in the Lowry method of protein determination

A simple technique for eliminating interference by detergents in the Lowry method of protein determination

The presence of nonionic and cationic detergents interfered in the Lowry method of protein estimation by causing precipitate formation. The addition of 0.5% sodium dodecylsulphate in the alkali reagent prevented this precipitation without affecting colour development, and allowed the method to be us...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Analytical biochemistry 1975-03, Vol.64 (1), p.136-141
Hauptverfasser: Dulley, J.R., Grieve, P.A.
Format: Artikel
Sprache:eng
Schlagworte:
Chemical Precipitation
Deoxycholic Acid
Polyethylene Glycols
Serum Albumin, Bovine - analysis
Sodium Dodecyl Sulfate
Surface-Active Agents
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The presence of nonionic and cationic detergents interfered in the Lowry method of protein estimation by causing precipitate formation. The addition of 0.5% sodium dodecylsulphate in the alkali reagent prevented this precipitation without affecting colour development, and allowed the method to be used on detergent treated membrane preparations.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(75)90415-7