A comparison of canine normal hepatic alkaline phosphatase and variant alkaline phosphatase of serum and liver

The isoenzyme of alkaline phosphatase from normal liver, the corticosteroid induced isoenzyme of alkaline phosphatase from serum and liver and a hepatocellular variant isoenzyme of alkaline phosphatase induced by lymphosarcoma have been partially purified and their the present modification incorpora...

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Veröffentlicht in:	Clinica chimica acta 1975-07, Vol.62 (1), p.137-142
Hauptverfasser:	Hoffmann, W E, Dorner, J L
Format:	Artikel
Sprache:	eng
Schlagworte:	Adrenal Cortex Diseases - enzymology Adrenal Cortex Hormones - pharmacology Alkaline Phosphatase - blood Alkaline Phosphatase - metabolism Animals Carcinoma, Hepatocellular - enzymology Dogs Drug Stability Enzyme Induction - drug effects Hot Temperature Hydrogen-Ion Concentration Isoenzymes - blood Isoenzymes - metabolism Kinetics Leucine - pharmacology Liver - drug effects Liver - enzymology Liver Neoplasms Lymphoma, Non-Hodgkin - enzymology Neoplasm Metastasis Phenylalanine - pharmacology Time Factors
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Zusammenfassung:	The isoenzyme of alkaline phosphatase from normal liver, the corticosteroid induced isoenzyme of alkaline phosphatase from serum and liver and a hepatocellular variant isoenzyme of alkaline phosphatase induced by lymphosarcoma have been partially purified and their the present modification incorporates Polybrene into buffer to eliminate this heparin interference. The proposed method shown excellent agreement with a reference procedure based on clottable protein, and excellent day-to-day precision (C.V.3.5%). The present method is easily adaptable to semi-automated measurements.
ISSN:	0009-8981