Fluorometric analysis of epinephrine and norepinephrine

Fluorometric analysis of epinephrine and norepinephrine

A modification of the classic trihydroxyindole method for the fluorometric estimation of epinephrine and norepinephrine is presented. Assay procedures for each catecholamine were separately optimized, significantly increasing the sensitivity and specificity of the procedures. Methodology has been si...

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Veröffentlicht in:Analytical biochemistry 1975-04, Vol.64 (2), p.578-587
Hauptverfasser: Campuzano, Helen C., Wilkerson, James E., Horvath, Steven M.
Format: Artikel
Sprache:eng
Schlagworte:
Acetates
Drug Stability
Epinephrine - analysis
Evaluation Studies as Topic
Humans
Hydrochloric Acid
Hydrogen-Ion Concentration
Indicators and Reagents
Methods
Norepinephrine - analysis
Perchlorates
Spectrometry, Fluorescence
Time Factors
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Zusammenfassung:A modification of the classic trihydroxyindole method for the fluorometric estimation of epinephrine and norepinephrine is presented. Assay procedures for each catecholamine were separately optimized, significantly increasing the sensitivity and specificity of the procedures. Methodology has been simplified through the use of intersolution calibration, making this assay suitable for use in research or clinical laboratories. Performance of both epinephrine and norepinephrine assays are possible on 1 ml of deproteinated plasma or urine using these procedures.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(75)90470-4