In vitro progesterone binding to Xenopus laevis oocytes

In vitro progesterone binding to Xenopus laevis oocytes

The melanosome fraction of Xenopus laevis oocytes was found to bind progesterone specifically. The binding is of high affinity and low capacity and displays a mean dissociation constant K d = 5.8 ± 3.2 × 10 −8 M at 4°C. Preliminary characterization of the receptor was performed and experiments attem...

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Veröffentlicht in:General and comparative endocrinology 1975-03, Vol.25 (3), p.339-345
Hauptverfasser: Bellé, R., Schorderet-Slatkine, S., Drury, K.C., Ozon, R.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Binding, Competitive
Catalysis
Deoxyribonucleases
Female
In Vitro Techniques
Oocytes - metabolism
Oocytes - ultrastructure
Ovum - ultrastructure
Progesterone - metabolism
Pronase
Protein Binding
Receptors, Cell Surface
Ribonucleases
Subcellular Fractions - metabolism
Tritium
Trypsin
Xenopus - metabolism
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Zusammenfassung:The melanosome fraction of Xenopus laevis oocytes was found to bind progesterone specifically. The binding is of high affinity and low capacity and displays a mean dissociation constant K d = 5.8 ± 3.2 × 10 −8 M at 4°C. Preliminary characterization of the receptor was performed and experiments attempting to assign a biological role in relation to oocyte maturation are discussed.
ISSN:0016-6480
1095-6840
DOI:10.1016/0016-6480(75)90124-0