Comparison of methods for the estimation of trypsin in duodenal fluid
Two spectrophotometric methods (amidase and esterase) and a titrimetric method (esterase) for estimating trypsin in human duodenal juice were compared. The results indicated that any of the three procedures was suitable for determining relative changes in trypsin levels although only the amidase met...
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| Veröffentlicht in: | Clinica chimica acta 1975-01, Vol.58 (2), p.165-171 |
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| container_title | Clinica chimica acta |
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| creator | Barns, R.J. Elmslie, R.G. |
| description | Two spectrophotometric methods (amidase and esterase) and a titrimetric method (esterase) for estimating trypsin in human duodenal juice were compared. The results indicated that any of the three procedures was suitable for determining relative changes in trypsin levels although only the amidase method gave a measure of the total tryptic activity present.
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R.J. Barns, University of Adelaide, Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000, Australia [t ] |
| doi_str_mv | 10.1016/S0009-8981(75)80008-8 |
| format | Article |
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*
R.J. Barns, University of Adelaide, Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000, Australia [t ]</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/S0009-8981(75)80008-8</identifier><identifier>PMID: 235383</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amidohydrolases - analysis ; Duodenum - enzymology ; Esterases - analysis ; Humans ; Hydrogen-Ion Concentration ; Intestinal Secretions - enzymology ; Methods ; Spectrophotometry, Ultraviolet ; Trypsin - analysis</subject><ispartof>Clinica chimica acta, 1975-01, Vol.58 (2), p.165-171</ispartof><rights>1975 Elsevier Scientific Publishing Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-abf4ac332346934748534a072244a38c0cf9fa58effdc2d697af82b0144468d93</citedby><cites>FETCH-LOGICAL-c359t-abf4ac332346934748534a072244a38c0cf9fa58effdc2d697af82b0144468d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0009-8981(75)80008-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/235383$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barns, R.J.</creatorcontrib><creatorcontrib>Elmslie, R.G.</creatorcontrib><title>Comparison of methods for the estimation of trypsin in duodenal fluid</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Two spectrophotometric methods (amidase and esterase) and a titrimetric method (esterase) for estimating trypsin in human duodenal juice were compared. The results indicated that any of the three procedures was suitable for determining relative changes in trypsin levels although only the amidase method gave a measure of the total tryptic activity present.
*
R.J. Barns, University of Adelaide, Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000, Australia [t ]</description><subject>Amidohydrolases - analysis</subject><subject>Duodenum - enzymology</subject><subject>Esterases - analysis</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Intestinal Secretions - enzymology</subject><subject>Methods</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Trypsin - analysis</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKAzEUhoN4q9U3UJiV6GI0t5kkK5FSL1Bwoa5DmguNzExqMiP07U07xa1w4HD4_3P7ALhC8A5BVN-_QwhFyQVHN6y65bniJT8AE8QZKQkV-BBM_iyn4Cylr1xSWKMTcIxJRTiZgPkstGsVfQpdEVzR2n4VTCpciEW_soVNvW9V70e1j5t18l2RwwzB2E41hWsGb87BkVNNshf7PAWfT_OP2Uu5eHt-nT0uSk0q0Zdq6ajShGBCa0Eoo7wiVEGGMaWKcA21E05V3DpnNDa1YMpxvISIUlpzI8gUXI9z1zF8D_k42fqkbdOozoYhSY6ZYAhtjdVo1DGkFK2T65gfiRuJoNzSkzt6cotGskru6Eme-y73C4Zla81f14gryw-jbPOTP95GmbS3nbbGR6t7aYL_Z8EvSbB-cA</recordid><startdate>19750101</startdate><enddate>19750101</enddate><creator>Barns, R.J.</creator><creator>Elmslie, R.G.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19750101</creationdate><title>Comparison of methods for the estimation of trypsin in duodenal fluid</title><author>Barns, R.J. ; Elmslie, R.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-abf4ac332346934748534a072244a38c0cf9fa58effdc2d697af82b0144468d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Amidohydrolases - analysis</topic><topic>Duodenum - enzymology</topic><topic>Esterases - analysis</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Intestinal Secretions - enzymology</topic><topic>Methods</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Trypsin - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barns, R.J.</creatorcontrib><creatorcontrib>Elmslie, R.G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barns, R.J.</au><au>Elmslie, R.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of methods for the estimation of trypsin in duodenal fluid</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1975-01-01</date><risdate>1975</risdate><volume>58</volume><issue>2</issue><spage>165</spage><epage>171</epage><pages>165-171</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Two spectrophotometric methods (amidase and esterase) and a titrimetric method (esterase) for estimating trypsin in human duodenal juice were compared. The results indicated that any of the three procedures was suitable for determining relative changes in trypsin levels although only the amidase method gave a measure of the total tryptic activity present.
*
R.J. Barns, University of Adelaide, Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000, Australia [t ]</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>235383</pmid><doi>10.1016/S0009-8981(75)80008-8</doi><tpages>7</tpages></addata></record> |
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| ispartof | Clinica chimica acta, 1975-01, Vol.58 (2), p.165-171 |
| issn | 0009-8981 1873-3492 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Amidohydrolases - analysis Duodenum - enzymology Esterases - analysis Humans Hydrogen-Ion Concentration Intestinal Secretions - enzymology Methods Spectrophotometry, Ultraviolet Trypsin - analysis |
| title | Comparison of methods for the estimation of trypsin in duodenal fluid |
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