Comparison of methods for the estimation of trypsin in duodenal fluid

Comparison of methods for the estimation of trypsin in duodenal fluid

Two spectrophotometric methods (amidase and esterase) and a titrimetric method (esterase) for estimating trypsin in human duodenal juice were compared. The results indicated that any of the three procedures was suitable for determining relative changes in trypsin levels although only the amidase met...

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Veröffentlicht in:Clinica chimica acta 1975-01, Vol.58 (2), p.165-171
Hauptverfasser: Barns, R.J., Elmslie, R.G.
Format: Artikel
Sprache:eng
Schlagworte:
Amidohydrolases - analysis
Duodenum - enzymology
Esterases - analysis
Humans
Hydrogen-Ion Concentration
Intestinal Secretions - enzymology
Methods
Spectrophotometry, Ultraviolet
Trypsin - analysis
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Zusammenfassung:Two spectrophotometric methods (amidase and esterase) and a titrimetric method (esterase) for estimating trypsin in human duodenal juice were compared. The results indicated that any of the three procedures was suitable for determining relative changes in trypsin levels although only the amidase method gave a measure of the total tryptic activity present. * R.J. Barns, University of Adelaide, Department of Surgery, Royal Adelaide Hospital, Adelaide, South Australia, 5000, Australia [t ]
ISSN:0009-8981
1873-3492
DOI:10.1016/S0009-8981(75)80008-8