Oxidation of reduced pyridine nucleotides in Pasteurella tularensis: II. Separation of diaphorases and transhydrogenases

Oxidation of reduced pyridine nucleotides in Pasteurella tularensis: II. Separation of diaphorases and transhydrogenases

Four diaphorases have been separated from sonicates of Pasteurella tularensis. A DPNH-specific diaphorase was obtained from deoxycholate extracts of the particulate fraction. It was stimulated by menadione. Three diaphorases were obtained from the soluble fraction: (A) a TPN-specific diaphorase, not...

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Veröffentlicht in:Biochimica et biophysica acta 1961-03, Vol.48 (1), p.85-92
Hauptverfasser: Robinson, Diana A., Mills, R.C.
Format: Artikel
Sprache:eng
Schlagworte:
Dihydrolipoamide Dehydrogenase
Francisella tularensis - metabolism
NADP Transhydrogenases
Nucleotides
Old Medline
Oxidation-Reduction
Oxidoreductases - chemistry
Pyridines
Retinal Degeneration
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Zusammenfassung:Four diaphorases have been separated from sonicates of Pasteurella tularensis. A DPNH-specific diaphorase was obtained from deoxycholate extracts of the particulate fraction. It was stimulated by menadione. Three diaphorases were obtained from the soluble fraction: (A) a TPN-specific diaphorase, not stimulated by menadione, which had no detectable transhydrogenase activity. (B) A DPNH-specific diaphorase, stimulated by menadione, which had some DPNH-3-acetylpyridine-DPN but no TPNH-DPN transhydrogenase activity. (C) A non-specific diaphorase having high TPNH-DPN, and low DPNH-3-acetylpyridine-DPN transhydrogenase activity.
ISSN:0006-3002
1878-2434
DOI:10.1016/0006-3002(61)90518-2