Effect of Growth Rate and Simian Adenovirus-7 Transformation on In vitro 67Ga Binding to Hamster Embryo Cells

The influence of growth rate and malignant transformation on 67Ga uptake was studied using cultured hamster embryo fibroblasts. Normalnonconfluent cells in log phase of growth bound approximately twice as much isotope as did confluent cells in a plateau phase of growth. In contrast, cells transformed by simian adenovirus-7 (SA-7), WHICH WERE ALSO UNDERGOING LOG GROWTH, BOUND ALMOST NO 67Ga. These results suggest that, although the rate of cellular proliferation may influence tissue affinity for 67Ga, other factors must also be considered when studying the effect of malignant transformation.