Techniques for rapid biochemical screening of large numbers of cell clones
Mutant cell lines derived from a variety of organisms can be isolated when a sufficient number of clones are screened. We describe techniques which can be used to clone up to 10 4 cells/h. The construction of a simple multiple pipet is outlined and its operation in conjunction with multitest culture...
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| Veröffentlicht in: | Experimental cell research 1975-02, Vol.90 (2), p.249-252 |
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| container_end_page | 252 |
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| container_issue | 2 |
| container_start_page | 249 |
| container_title | Experimental cell research |
| container_volume | 90 |
| creator | Brenner, M. Tisdale, D. Loomis, W.F. |
| description | Mutant cell lines derived from a variety of organisms can be isolated when a sufficient number of clones are screened. We describe techniques which can be used to clone up to 10
4 cells/h. The construction of a simple multiple pipet is outlined and its operation in conjunction with multitest culture trays and an efficient replicator is described. The techniques permit screening of any cells able to grow clonally in culture. |
| doi_str_mv | 10.1016/0014-4827(75)90313-4 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0014-4827 |
| ispartof | Experimental cell research, 1975-02, Vol.90 (2), p.249-252 |
| issn | 0014-4827 1090-2422 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82735610 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Cell Line Clone Cells - analysis Culture Techniques - instrumentation Methods Myxomycetes - analysis Time Factors |
| title | Techniques for rapid biochemical screening of large numbers of cell clones |
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