Techniques for rapid biochemical screening of large numbers of cell clones

Techniques for rapid biochemical screening of large numbers of cell clones

Mutant cell lines derived from a variety of organisms can be isolated when a sufficient number of clones are screened. We describe techniques which can be used to clone up to 10 4 cells/h. The construction of a simple multiple pipet is outlined and its operation in conjunction with multitest culture...

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Veröffentlicht in:Experimental cell research 1975-02, Vol.90 (2), p.249-252
Hauptverfasser: Brenner, M., Tisdale, D., Loomis, W.F.
Format: Artikel
Sprache:eng
Schlagworte:
Cell Line
Clone Cells - analysis
Culture Techniques - instrumentation
Methods
Myxomycetes - analysis
Time Factors
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Zusammenfassung:Mutant cell lines derived from a variety of organisms can be isolated when a sufficient number of clones are screened. We describe techniques which can be used to clone up to 10 4 cells/h. The construction of a simple multiple pipet is outlined and its operation in conjunction with multitest culture trays and an efficient replicator is described. The techniques permit screening of any cells able to grow clonally in culture.
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(75)90313-4