A simple method for the elimination of platelets from the lymphocyte-platelet mixture by sucrose

A simple method for the elimination of platelets from the lymphocyte-platelet mixture by sucrose

A method for the separation of lymphocytes from human blood by Ficoll-Hypaque density centrifugation, with removal of platelets by a single sucrose solution with a density of 65%, is presented. The Ficoll-Hypaque-sucrose method gives a good yield of lymphocytes, high viability and purity, and relati...

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Veröffentlicht in:American journal of clinical pathology 1975-02, Vol.63 (2), p.237-239
Hauptverfasser: Chanarat, P, Chiewsilp, P
Format: Artikel
Sprache:eng
Schlagworte:
Anticoagulants
Blood Platelets
Centrifugation, Density Gradient - methods
Citrates
Edetic Acid
Heparin
Histocompatibility Testing
Humans
Lymphocytes
Sucrose
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container_end_page 239
container_issue 2
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container_title American journal of clinical pathology
container_volume 63
creator Chanarat, P
Chiewsilp, P
description A method for the separation of lymphocytes from human blood by Ficoll-Hypaque density centrifugation, with removal of platelets by a single sucrose solution with a density of 65%, is presented. The Ficoll-Hypaque-sucrose method gives a good yield of lymphocytes, high viability and purity, and relatively low contamination with erythrocytes and platelets.
doi_str_mv 10.1093/ajcp/63.2.237
format Article
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ispartof American journal of clinical pathology, 1975-02, Vol.63 (2), p.237-239
issn 0002-9173
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source MEDLINE; Oxford University Press Archive
subjects Anticoagulants
Blood Platelets
Centrifugation, Density Gradient - methods
Citrates
Edetic Acid
Heparin
Histocompatibility Testing
Humans
Lymphocytes
Sucrose
title A simple method for the elimination of platelets from the lymphocyte-platelet mixture by sucrose
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