A simple method for the elimination of platelets from the lymphocyte-platelet mixture by sucrose

A simple method for the elimination of platelets from the lymphocyte-platelet mixture by sucrose

A method for the separation of lymphocytes from human blood by Ficoll-Hypaque density centrifugation, with removal of platelets by a single sucrose solution with a density of 65%, is presented. The Ficoll-Hypaque-sucrose method gives a good yield of lymphocytes, high viability and purity, and relati...

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Veröffentlicht in:American journal of clinical pathology 1975-02, Vol.63 (2), p.237-239
Hauptverfasser: Chanarat, P, Chiewsilp, P
Format: Artikel
Sprache:eng
Schlagworte:
Anticoagulants
Blood Platelets
Centrifugation, Density Gradient - methods
Citrates
Edetic Acid
Heparin
Histocompatibility Testing
Humans
Lymphocytes
Sucrose
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Zusammenfassung:A method for the separation of lymphocytes from human blood by Ficoll-Hypaque density centrifugation, with removal of platelets by a single sucrose solution with a density of 65%, is presented. The Ficoll-Hypaque-sucrose method gives a good yield of lymphocytes, high viability and purity, and relatively low contamination with erythrocytes and platelets.
ISSN:0002-9173
1943-7722
DOI:10.1093/ajcp/63.2.237