IN VIVO AND IN VITRO BINDING OF ETHIONINE WITH NUCLEIC ACIDS

IN VIVO AND IN VITRO BINDING OF ETHIONINE WITH NUCLEIC ACIDS

The binding in vivo of ethionine with RNA, nuclear proteins, cytoplasmic proteins, and with DNA of rat liver, using high doses of carcinogen, is evidentiated. The binding in vitro to DNA is microsome-dependent; it is higher using polynucleotides as acceptors, under the same experimental conditions....

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Veröffentlicht in:GANN Japanese Journal of Cancer Research 1974/12/31, Vol.65(6), pp.507-511
Hauptverfasser: GRILLI, Sandro, FERRERI, Anna Maria, ROCCHI, Paola, PRODI, Giorgio
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cytoplasm - metabolism
DNA - metabolism
Ethionine
Ethionine - metabolism
Guanine - metabolism
in vitro and in vivo binding
In Vitro Techniques
Mechanism of carcinogenesis
Microsomes, Liver - metabolism
Nucleic acid
Polynucleotides - metabolism
Protein Binding
Proteins - metabolism
Rats
RNA - metabolism
RNA, Ribosomal - metabolism
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Zusammenfassung:The binding in vivo of ethionine with RNA, nuclear proteins, cytoplasmic proteins, and with DNA of rat liver, using high doses of carcinogen, is evidentiated. The binding in vitro to DNA is microsome-dependent; it is higher using polynucleotides as acceptors, under the same experimental conditions. Acid hydrolysis performed on DNA and poly-A after incubation in vitro leads to radioactive compounds, not coincident with 7-ethylguanine.
ISSN:0016-450X
DOI:10.20772/cancersci1959.65.6_507