Specific extraction of human serum albumin by partition in aqueous biphasic systems containing poly(ethylene glycol) bound ligand

Specific extraction of human serum albumin by partition in aqueous biphasic systems containing poly(ethylene glycol) bound ligand

Albumin has been specifically extracted from plasma by partition in aqueous biphasic systems containing dextran and PEG, the latter partly esterified with palmitic acid. In such a system albumin, due to high binding affinity for the palmitoyl residues, is concentrated in the PEG-rich phase.

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Bibliographische Detailangaben
Veröffentlicht in:Biochemical and biophysical research communications 1974-12, Vol.61 (4), p.1141-1146
Hauptverfasser: Shanbhag, V.P., Johansson, G.
Format: Artikel
Sprache:eng
Schlagworte:
Binding Sites
Countercurrent Distribution
Humans
Hydrogen-Ion Concentration
Methods
Polyethylene Glycols
Protein Binding
Serum Albumin - isolation & purification
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Zusammenfassung:Albumin has been specifically extracted from plasma by partition in aqueous biphasic systems containing dextran and PEG, the latter partly esterified with palmitic acid. In such a system albumin, due to high binding affinity for the palmitoyl residues, is concentrated in the PEG-rich phase.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(74)80402-X