A Study of Antibody Production in Vitro
Sieved spleen cells isolated from rabbits 2 or 3 days after an in vivo anamnestic stimulation have been studied. Synthesis of antibody in vitro has been measured by the incorporation of radioactive amino acid into carrier antibody-antigen precipitates formed in the supernatants. The effect of altera...
Gespeichert in:
| Veröffentlicht in: | The Journal of immunology (1950) 1960-03, Vol.84 (3), p.258-267 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 267 |
|---|---|
| container_issue | 3 |
| container_start_page | 258 |
| container_title | The Journal of immunology (1950) |
| container_volume | 84 |
| creator | Vaughan, John H Dutton, Anne H Dutton, Richard W George, Mariam Marston, Robert Q |
| description | Sieved spleen cells isolated from rabbits 2 or 3 days after an in vivo anamnestic stimulation have been studied. Synthesis of antibody in vitro has been measured by the incorporation of radioactive amino acid into carrier antibody-antigen precipitates formed in the supernatants. The effect of alterations of the culture medium have been investigated. The rate of antibody production tended to be constant in spleen cells isolated at 3 days, but was still increasing in cells isolated at 2 days. |
| doi_str_mv | 10.4049/jimmunol.84.3.258 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_82672921</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>82672921</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2878-8e47232e96a874ef9933b42ac2c8f835fa8ef117019108bade0d20c50c1639993</originalsourceid><addsrcrecordid>eNpFkDtPwzAURi0EoqXwA1hQFmBKuH7Edsaq4iVVAonHarmOQ10lMdiJov57glpgunc43xkOQucYMgasuNm4pulbX2eSZTQjuTxAU5znkHIO_BBNAQhJseBigk5i3AAAB8KO0QRTyTATcoqu58lL15fbxFfJvO3cyo__c_Blbzrn28S1ybvrgj9FR5Wuoz3b3xl6u7t9XTyky6f7x8V8mRoihUylZYJQYguupWC2KgpKV4xoQ4ysJM0rLW2FsQBcYJArXVooCZgcDOa0GOkZutp5P4P_6m3sVOOisXWtW-v7qCThghQEjyDegSb4GIOt1GdwjQ5bhUH91FG_dZRkiqqxzri52Mv7VWPL_8U-xwhc7oC1-1gPLlgVG13XI47VMAx_om81EG1m</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>82672921</pqid></control><display><type>article</type><title>A Study of Antibody Production in Vitro</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Vaughan, John H ; Dutton, Anne H ; Dutton, Richard W ; George, Mariam ; Marston, Robert Q</creator><creatorcontrib>Vaughan, John H ; Dutton, Anne H ; Dutton, Richard W ; George, Mariam ; Marston, Robert Q</creatorcontrib><description>Sieved spleen cells isolated from rabbits 2 or 3 days after an in vivo anamnestic stimulation have been studied. Synthesis of antibody in vitro has been measured by the incorporation of radioactive amino acid into carrier antibody-antigen precipitates formed in the supernatants. The effect of alterations of the culture medium have been investigated. The rate of antibody production tended to be constant in spleen cells isolated at 3 days, but was still increasing in cells isolated at 2 days.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.84.3.258</identifier><identifier>PMID: 13841478</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Antibodies ; Antibody Formation ; Humans ; In Vitro Techniques ; Old Medline</subject><ispartof>The Journal of immunology (1950), 1960-03, Vol.84 (3), p.258-267</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2878-8e47232e96a874ef9933b42ac2c8f835fa8ef117019108bade0d20c50c1639993</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13841478$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vaughan, John H</creatorcontrib><creatorcontrib>Dutton, Anne H</creatorcontrib><creatorcontrib>Dutton, Richard W</creatorcontrib><creatorcontrib>George, Mariam</creatorcontrib><creatorcontrib>Marston, Robert Q</creatorcontrib><title>A Study of Antibody Production in Vitro</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>Sieved spleen cells isolated from rabbits 2 or 3 days after an in vivo anamnestic stimulation have been studied. Synthesis of antibody in vitro has been measured by the incorporation of radioactive amino acid into carrier antibody-antigen precipitates formed in the supernatants. The effect of alterations of the culture medium have been investigated. The rate of antibody production tended to be constant in spleen cells isolated at 3 days, but was still increasing in cells isolated at 2 days.</description><subject>Antibodies</subject><subject>Antibody Formation</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Old Medline</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1960</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkDtPwzAURi0EoqXwA1hQFmBKuH7Edsaq4iVVAonHarmOQ10lMdiJov57glpgunc43xkOQucYMgasuNm4pulbX2eSZTQjuTxAU5znkHIO_BBNAQhJseBigk5i3AAAB8KO0QRTyTATcoqu58lL15fbxFfJvO3cyo__c_Blbzrn28S1ybvrgj9FR5Wuoz3b3xl6u7t9XTyky6f7x8V8mRoihUylZYJQYguupWC2KgpKV4xoQ4ysJM0rLW2FsQBcYJArXVooCZgcDOa0GOkZutp5P4P_6m3sVOOisXWtW-v7qCThghQEjyDegSb4GIOt1GdwjQ5bhUH91FG_dZRkiqqxzri52Mv7VWPL_8U-xwhc7oC1-1gPLlgVG13XI47VMAx_om81EG1m</recordid><startdate>19600301</startdate><enddate>19600301</enddate><creator>Vaughan, John H</creator><creator>Dutton, Anne H</creator><creator>Dutton, Richard W</creator><creator>George, Mariam</creator><creator>Marston, Robert Q</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19600301</creationdate><title>A Study of Antibody Production in Vitro</title><author>Vaughan, John H ; Dutton, Anne H ; Dutton, Richard W ; George, Mariam ; Marston, Robert Q</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2878-8e47232e96a874ef9933b42ac2c8f835fa8ef117019108bade0d20c50c1639993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1960</creationdate><topic>Antibodies</topic><topic>Antibody Formation</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Old Medline</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vaughan, John H</creatorcontrib><creatorcontrib>Dutton, Anne H</creatorcontrib><creatorcontrib>Dutton, Richard W</creatorcontrib><creatorcontrib>George, Mariam</creatorcontrib><creatorcontrib>Marston, Robert Q</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vaughan, John H</au><au>Dutton, Anne H</au><au>Dutton, Richard W</au><au>George, Mariam</au><au>Marston, Robert Q</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Study of Antibody Production in Vitro</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1960-03-01</date><risdate>1960</risdate><volume>84</volume><issue>3</issue><spage>258</spage><epage>267</epage><pages>258-267</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>Sieved spleen cells isolated from rabbits 2 or 3 days after an in vivo anamnestic stimulation have been studied. Synthesis of antibody in vitro has been measured by the incorporation of radioactive amino acid into carrier antibody-antigen precipitates formed in the supernatants. The effect of alterations of the culture medium have been investigated. The rate of antibody production tended to be constant in spleen cells isolated at 3 days, but was still increasing in cells isolated at 2 days.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>13841478</pmid><doi>10.4049/jimmunol.84.3.258</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1960-03, Vol.84 (3), p.258-267 |
| issn | 0022-1767 1550-6606 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82672921 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Antibodies Antibody Formation Humans In Vitro Techniques Old Medline |
| title | A Study of Antibody Production in Vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T01%3A49%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Study%20of%20Antibody%20Production%20in%20Vitro&rft.jtitle=The%20Journal%20of%20immunology%20(1950)&rft.au=Vaughan,%20John%20H&rft.date=1960-03-01&rft.volume=84&rft.issue=3&rft.spage=258&rft.epage=267&rft.pages=258-267&rft.issn=0022-1767&rft.eissn=1550-6606&rft_id=info:doi/10.4049/jimmunol.84.3.258&rft_dat=%3Cproquest_cross%3E82672921%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=82672921&rft_id=info:pmid/13841478&rfr_iscdi=true |