A Study of Antibody Production in Vitro
Sieved spleen cells isolated from rabbits 2 or 3 days after an in vivo anamnestic stimulation have been studied. Synthesis of antibody in vitro has been measured by the incorporation of radioactive amino acid into carrier antibody-antigen precipitates formed in the supernatants. The effect of altera...
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| Veröffentlicht in: | The Journal of immunology (1950) 1960-03, Vol.84 (3), p.258-267 |
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| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
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| Online-Zugang: | Volltext |
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| Zusammenfassung: | Sieved spleen cells isolated from rabbits 2 or 3 days after an in vivo anamnestic stimulation have been studied. Synthesis of antibody in vitro has been measured by the incorporation of radioactive amino acid into carrier antibody-antigen precipitates formed in the supernatants. The effect of alterations of the culture medium have been investigated. The rate of antibody production tended to be constant in spleen cells isolated at 3 days, but was still increasing in cells isolated at 2 days. |
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| ISSN: | 0022-1767 1550-6606 |
| DOI: | 10.4049/jimmunol.84.3.258 |