The occurrence of a transmethylation reaction not involving S-adenosylmethionine in the formation of ribothymidine in Bacillus subtilis transfer-RNA

The occurrence of a transmethylation reaction not involving S-adenosylmethionine in the formation of ribothymidine in Bacillus subtilis transfer-RNA

The bulk transfer-RNA of Bacillus subtilis contains approximately one thymine residue per molecule. The methyl group of the thymine does not become labeled when the organism is grown in the presence of [methyl- 14C]methionine, although other methylated bases of the B. subtilis tRNA do become labeled...

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Veröffentlicht in:Biochemical and biophysical research communications 1974-12, Vol.61 (4), p.1256-1261
Hauptverfasser: Romeo, Joseph M., Delk, Ann S., Rabinowitz, Jesse C.
Format: Artikel
Sprache:eng
Schlagworte:
Bacillus subtilis - enzymology
Chromatography, Thin Layer
Methionine - metabolism
Ribonucleosides - biosynthesis
RNA, Bacterial
RNA, Transfer
S-Adenosylmethionine - metabolism
Thymidine - biosynthesis
tRNA Methyltransferases - metabolism
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Zusammenfassung:The bulk transfer-RNA of Bacillus subtilis contains approximately one thymine residue per molecule. The methyl group of the thymine does not become labeled when the organism is grown in the presence of [methyl- 14C]methionine, although other methylated bases of the B. subtilis tRNA do become labeled.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(74)80419-5