Separation of Isoenzymes by Partition in Aqueous Polymeric Two‐Phase Systems

A method for the separation of isoenzymes by counter‐current distribution in aqueous biphasic systems is described. The phase systems used contained dextran and trimethylamino‐poly(ethyleneglycol). The method has been tested with three isoenzyme systems, namely, lactic dehydrogenase, malate dehydrog...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	European journal of biochemistry 1974-10, Vol.48 (1), p.63-69
Hauptverfasser:	BLOMQUIST, Göran, HARTMAN, Annika, SHANBHAG, Vasant, JOHANSSON, Göte
Format:	Artikel
Sprache:	eng
Schlagworte:	Countercurrent Distribution Electrophoresis, Polyacrylamide Gel Isoelectric Focusing Isoenzymes - isolation & purification L-Lactate Dehydrogenase - isolation & purification Malate Dehydrogenase - isolation & purification Phosphopyruvate Hydratase - isolation & purification Polyethylene Glycols Quaternary Ammonium Compounds Water
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A method for the separation of isoenzymes by counter‐current distribution in aqueous biphasic systems is described. The phase systems used contained dextran and trimethylamino‐poly(ethyleneglycol). The method has been tested with three isoenzyme systems, namely, lactic dehydrogenase, malate dehydrogenase and enolase. Separation of isoenzymes in extracts of tissue or cells as well as in a mixture of commercial isoenzyme preparations were successfully achieved by this technique.
ISSN:	0014-2956 1432-1033
DOI:	10.1111/j.1432-1033.1974.tb03743.x