Focal differences in lipid metabolism of the young pig aorta: Part 2. Synthesis from [2- 14C]ethanolamine and [1,2- 14C]choline

The ability of normal young pig aortic tissue to synthesize phospholipids from [2- 14C]ethanolamine and [1,2- 14C]choline, in vitro, has been examined in areas of focal Evans Blue accumulation (blue areas) and adjacent areas of no dye accumulation (white areas). Incorporation of [2- 14C]ethanolamine into total lipid was linear for 3 h of incubation in both blue and white areas. At 3 h, ethanolamine incorporation into phosphatidyl ethanolamine was significantly less in blue than in white areas. [1,2- 14C]Choline incorporation into total lipid was linear for 3 h of incubation in blue areas but not in white areas. At 30 min, choline incorporation into phosphatidyl choline was significantly less in blue than in white areas; at 1 h choline incorporation into phosphatidyl choline was similar in blue and white areas, while after 3 h of incubation incorporation was significantly greater in blue than in white areas. With both [2- 14C]ethanolamine and [1,2- 14C]choline, the percentage distribution of label among individual phospholipids was similar in blue and white areas. Phospholipid content of blue and white areas was similar. The results presented demonstrate further focal metabolic differences within the same geographical region of the normal young pig aorta.