Amino acid sequence of a ( 32P)phosphopeptide from pig liver pyruvate kinase phosphorylated by cyclic 3′,5′-AMP-stimulated protein kinase and γ-( 32P)ATP

Pig liver pyruvate kinase (type L) was 32P-labelled by incubation with ( 32P)ATP and cyclic 3′,5′-AMP-stimulated protein kinase from the same source. One major ( 32P)phosphopeptide was isolated from a peptic hydrolysate of the enzyme. Its amino acid sequence was Leu-Arg-Arg-Ala-( 32P)SerP-Leu.

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 1974-11, Vol.61 (2), p.559-563
Hauptverfasser:	Hjelmquist, Gunilla, Andersson, Jill, Edlund, Bror, Engström, Lorentz
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate Amino Acid Sequence Animals Chromatography, Gel Chromatography, Ion Exchange Cyclic AMP - pharmacology Enzyme Activation - drug effects Liver - enzymology Molecular Weight Pepsin A Phosphopeptides - metabolism Phosphorus Radioisotopes Protein Kinases - metabolism Pyruvate Kinase - isolation & purification Pyruvate Kinase - metabolism Swine
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Pig liver pyruvate kinase (type L) was 32P-labelled by incubation with ( 32P)ATP and cyclic 3′,5′-AMP-stimulated protein kinase from the same source. One major ( 32P)phosphopeptide was isolated from a peptic hydrolysate of the enzyme. Its amino acid sequence was Leu-Arg-Arg-Ala-( 32P)SerP-Leu.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(74)90993-0