Stability of ribonucleic acid double-stranded helices
The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of Δ G, Δ H, a...
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| Veröffentlicht in: | Journal of molecular biology 1974-07, Vol.86 (4), p.843-853 |
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| container_end_page | 853 |
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| container_issue | 4 |
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| container_title | Journal of molecular biology |
| container_volume | 86 |
| creator | Borer, Philip N. Dengler, Barbara Tinoco, Ignacio Uhlenbeck, Olke C. |
| description | The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of Δ
G, Δ
H, and Δ
S of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the
T
m of any RNA double helix of known sequence. |
| doi_str_mv | 10.1016/0022-2836(74)90357-X |
| format | Article |
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G, Δ
H, and Δ
S of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the
T
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G, Δ
H, and Δ
S of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the
T
m of any RNA double helix of known sequence.</description><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic Acid Denaturation</subject><subject>Oligonucleotides</subject><subject>RNA</subject><subject>Spectrophotometry</subject><subject>Temperature</subject><subject>Thermodynamics</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLw0AQxxdRaq1-A4WcRA_Rfe_mIkjxBQUPKvS2bHYnuJImdTcR-u1NbOnR0wz8H8P8EDon-IZgIm8xpjSnmskrxa8LzITKlwdoSrAuci2ZPkTTveUYnaT0hTEWjOsJmnBO1RCYIvHW2TLUodtkbZXFULZN72oILrMu-My3fVlDnrpoGw8--4Q6OEin6KiydYKz3Zyhj8eH9_lzvnh9epnfL3LHFe9y4hwhTGnpKbaWi2HhtlCSEacKXVFaSMqhdCUGgcFrS5l0VAARuKKq5GyGLre969h-95A6swrJQV3bBto-GU0lVoSJwci3RhfblCJUZh3DysaNIdiMtMyIwowojOLmj5ZZDrGLXX9frsDvQzs8g3631WF48idANMkFaBz4EMF1xrfh_wO_PpZ37w</recordid><startdate>19740715</startdate><enddate>19740715</enddate><creator>Borer, Philip N.</creator><creator>Dengler, Barbara</creator><creator>Tinoco, Ignacio</creator><creator>Uhlenbeck, Olke C.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19740715</creationdate><title>Stability of ribonucleic acid double-stranded helices</title><author>Borer, Philip N. ; Dengler, Barbara ; Tinoco, Ignacio ; Uhlenbeck, Olke C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-1cc113786d20aa4586d4a97631c798f229624ebcb0e50ed8a236c25e150f27b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic Acid Denaturation</topic><topic>Oligonucleotides</topic><topic>RNA</topic><topic>Spectrophotometry</topic><topic>Temperature</topic><topic>Thermodynamics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Borer, Philip N.</creatorcontrib><creatorcontrib>Dengler, Barbara</creatorcontrib><creatorcontrib>Tinoco, Ignacio</creatorcontrib><creatorcontrib>Uhlenbeck, Olke C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Borer, Philip N.</au><au>Dengler, Barbara</au><au>Tinoco, Ignacio</au><au>Uhlenbeck, Olke C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stability of ribonucleic acid double-stranded helices</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1974-07-15</date><risdate>1974</risdate><volume>86</volume><issue>4</issue><spage>843</spage><epage>853</epage><pages>843-853</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of Δ
G, Δ
H, and Δ
S of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the
T
m of any RNA double helix of known sequence.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>4427357</pmid><doi>10.1016/0022-2836(74)90357-X</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2836 |
| ispartof | Journal of molecular biology, 1974-07, Vol.86 (4), p.843-853 |
| issn | 0022-2836 1089-8638 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82607135 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Base Sequence Binding Sites Nucleic Acid Conformation Nucleic Acid Denaturation Oligonucleotides RNA Spectrophotometry Temperature Thermodynamics |
| title | Stability of ribonucleic acid double-stranded helices |
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