Stability of ribonucleic acid double-stranded helices

Stability of ribonucleic acid double-stranded helices

The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of Δ G, Δ H, a...

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Veröffentlicht in:Journal of molecular biology 1974-07, Vol.86 (4), p.843-853
Hauptverfasser: Borer, Philip N., Dengler, Barbara, Tinoco, Ignacio, Uhlenbeck, Olke C.
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
Binding Sites
Nucleic Acid Conformation
Nucleic Acid Denaturation
Oligonucleotides
RNA
Spectrophotometry
Temperature
Thermodynamics
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Zusammenfassung:The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of Δ G, Δ H, and Δ S of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the T m of any RNA double helix of known sequence.
ISSN:0022-2836
1089-8638
DOI:10.1016/0022-2836(74)90357-X