Lipids of higher fungi. II. The nature of cerebrins and cerebrosides from the mushroom Clitocybe tabescens Scop

Four cerebrins (ceramides) and two cerebrosides were isolated from the mushroom Clitocybe tabescens by combining column chromatography and preparative thin-layer chromatography. Quantification of the amide-linked nonhydroxy and hydroxy fatty acids (NFA and HFA) was obtained by gas—liquid chromatography. The nature of the long-chain bases was determined by thin-layer chromatography. The major acids in cerebrins were: NFA 16:0, 17:0, 18:X, 22:0 and HFA h16:0, h18:0, h22:0, h24:0. In cerebrosides predominated 17:0, 22:0 and h16:0. The component long-chain bases in all samples were sphingosines, dihydrosphingosines, phytosphingosines and unidentified more polar bases. Glucose was detected as the sole sugar constituent in cerebrosides.