The use of homogenization procedures for extracting nuclei from plant cells

A method is described by which relatively large yields of plant nuclei can be isolated. The main feature of the technique is the arrangement to maintain a fairly large clearance, during homogenization, between the rotating pestle and the containing tube. It is believed that this form of homogenization does not grind the tissue but acts by bursting the cells, so reducing both the damage done to cell organelles and the danger of diffusion artifacts. The significance of this method in the study of the localization of DNA will be considered in a subsequent communication.