Evaluation of Specific Protein Analysis in a Non-Teaching Hospital Laboratory

A retrospective study has been made to assess the value of specific protein analysis in a routine laboratory. The use of cellulose acetate electrophoresis, immunoelectrophoresis, and single radial immunodiffusion has been discussed with reference to the source of the request for the investigation and the yield of abnormal results. A scheme for the investigation of abnormal proteins is presented with a discussion of the problem of diagnosing myeloma as distinct from identifying an abnormal protein. The occurrence of double-zones on Tri-Partigen plates (Hoechst Pharm.) may be an early indication of myeloma, but does occur in other conditions. Levels of immunoglobulins in the geriatric age group have been shown to be similar to that of non-geriatric adults. Specific protein analysis has been shown to be of limited value in rheumatoid arthritis.