Glutathione in the isolated perfused rabbit kidney

Perfusion of the isolated rabbit kidney at normothermia leads to a marked depletion in the concentration of reduced glutathione in the kidney tissue. After 90 min of perfusion at 36 ± 1°C, the concentration of GSH found in the renal cortex is more than 80% lower than the levels found in fresh tissue...

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Veröffentlicht in:The Journal of surgical research 1974-10, Vol.17 (4), p.228-231
Hauptverfasser: Leibach, Fredrick H., Fonteles, Manasses C., Pillion, Dennis, Karow, Armand M.
Format: Artikel
Sprache:eng
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Zusammenfassung:Perfusion of the isolated rabbit kidney at normothermia leads to a marked depletion in the concentration of reduced glutathione in the kidney tissue. After 90 min of perfusion at 36 ± 1°C, the concentration of GSH found in the renal cortex is more than 80% lower than the levels found in fresh tissue. Comparable perfusion under hypothermic conditions reduced the amount of glutathione depletion considerably; however, after 90 min of perfusion at 15 ± 1°C, renal cortex GSH levels were still nearly 50% lower than the levels found in fresh tissue. It was found that the addition of GSH to the perfusate completely alleviated this glutathione-depletion phenomenon.
ISSN:0022-4804
1095-8673
DOI:10.1016/0022-4804(74)90113-9