A modified procedure for the automatic analysis of amino acids
A simplified scheme for the automatic analysis of amino acids has been developed. It employs a single column, 133 × 0.9 cm, of Dowex 50X12, 25–32 μ (ground), which is eluted with a continuous gradient. The gradient is produced by a nine-chambered device (Varigrad) which allows a wide variety of elut...
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| Veröffentlicht in: | Analytical biochemistry 1960-11, Vol.1 (3), p.187-201 |
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| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
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| Online-Zugang: | Volltext |
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| Zusammenfassung: | A simplified scheme for the automatic analysis of amino acids has been developed. It employs a single column, 133 × 0.9 cm, of Dowex 50X12, 25–32 μ (ground), which is eluted with a continuous gradient. The gradient is produced by a nine-chambered device (Varigrad) which allows a wide variety of elution schemes to be constructed. The procedure described allows the complete analysis of a protein hydrolyzate, including collagen, in 24 hr using a single sample. It is also suitable for many other biological amino acid mixtures. |
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| ISSN: | 0003-2697 1096-0309 |
| DOI: | 10.1016/0003-2697(60)90045-2 |