The radiometric assay of α-amylase activity
A method is presented for coupling 1 2 5 I to the chromophore of an insoluble amylopectin using Chloramine T. The radioactive starch can be used to assay α-amylolytic activity. The assay is convenient, sensitive, inexpensive and requires only 5 μ1 volumes of enzyme solution. Conditions for the proce...
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Veröffentlicht in: | Clinica chimica acta 1974-11, Vol.56 (3), p.291-295 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A method is presented for coupling
1 2 5 I to the chromophore of an insoluble amylopectin using Chloramine T. The radioactive starch can be used to assay α-amylolytic activity. The assay is convenient, sensitive, inexpensive and requires only 5 μ1 volumes of enzyme solution. Conditions for the procedure are given and the results with human pancreatic α-amylase are discussed. |
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ISSN: | 0009-8981 1873-3492 |
DOI: | 10.1016/0009-8981(74)90141-7 |