The radiometric assay of α-amylase activity

A method is presented for coupling 1 2 5 I to the chromophore of an insoluble amylopectin using Chloramine T. The radioactive starch can be used to assay α-amylolytic activity. The assay is convenient, sensitive, inexpensive and requires only 5 μ1 volumes of enzyme solution. Conditions for the proce...

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Veröffentlicht in:Clinica chimica acta 1974-11, Vol.56 (3), p.291-295
1. Verfasser: Aw, S.E.
Format: Artikel
Sprache:eng
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Zusammenfassung:A method is presented for coupling 1 2 5 I to the chromophore of an insoluble amylopectin using Chloramine T. The radioactive starch can be used to assay α-amylolytic activity. The assay is convenient, sensitive, inexpensive and requires only 5 μ1 volumes of enzyme solution. Conditions for the procedure are given and the results with human pancreatic α-amylase are discussed.
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(74)90141-7