Identification of sparganum growth factor by a radioreceptor assay for growth hormone
The growth hormone-like substance of Spirometra mansonoides in the incubation medium of the worm as well as in serum from infested rats was studied with the radio-receptor assay (RRA) for growth hormone (GH). Sparganum growth factor (SGF) inhibited the binding of hGH tracer to the rabbit liver recep...
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Veröffentlicht in: | Biochem. Biophys. Res. Commun., v. 59, no. 3, pp. 1062-1068 v. 59, no. 3, pp. 1062-1068, 1974-08, Vol.59 (3), p.1062-1068 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The growth hormone-like substance of Spirometra mansonoides in the incubation medium of the worm as well as in serum from infested rats was studied with the radio-receptor assay (RRA) for growth hormone (GH). Sparganum growth factor (SGF) inhibited the binding of hGH tracer to the rabbit liver receptor in a parallel manner to human GH. Serum concentrations of SGF in normal rats increased to a level equivalent to 1 μg/ml hGH eight days after subcutaneous implantation of 50 worms. Upon gel filtration in G-100 Sephadex, SGF eluted in the fractions corresponding to a molecular weight of approximately 70,000. The Rf of SGF upon polyocrylamide disc gel electrophoresis was 0.32 and after gel isoelectrofocusing, the growth factor was detected in gel segment of pH 6.0. |
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ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/S0006-291X(74)80087-2 |