Routine measurements of liver and bone alkaline phosphatase in human serum: Differential inhibition by l-phenylalanine and carbamide (urea) on the LKB 8600 reaction rate analyzer

Routine measurements of liver and bone alkaline phosphatase in human serum: Differential inhibition by l-phenylalanine and carbamide (urea) on the LKB 8600 reaction rate analyzer

A method suitable for routine determination of liver and bone alkaline phosphatase (EC 3.1.3.1) on the LKB 8600 is described. Experimental data on differential inhibition by l-phenylalanine, 10 mmoles/1 and carbamide, 3.81 moles/1 under strictly controlled conditions on the LKB 8600 are given. Data...

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Veröffentlicht in:Clinica chimica acta 1974-06, Vol.53 (3), p.281-290
Hauptverfasser: Gerhardt, W., Nielsen, M.Lykkegaard, Vagn Nielsen, O., Olsen, J.S., Statland, B.E.
Format: Artikel
Sprache:eng
Schlagworte:
Alkaline Phosphatase - antagonists & inhibitors
Alkaline Phosphatase - blood
Alkaline Phosphatase - metabolism
Autoanalysis
Bone and Bones - enzymology
Child, Preschool
Drug Stability
Evaluation Studies as Topic
Humans
Jejunum - enzymology
Kinetics
Liver - enzymology
Mathematics
Methods
Phenylalanine - pharmacology
Temperature
Time Factors
Urea - pharmacology
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Zusammenfassung:A method suitable for routine determination of liver and bone alkaline phosphatase (EC 3.1.3.1) on the LKB 8600 is described. Experimental data on differential inhibition by l-phenylalanine, 10 mmoles/1 and carbamide, 3.81 moles/1 under strictly controlled conditions on the LKB 8600 are given. Data on the stability of the tissue alkaline phosphatase extracts and on the precision of the analytical system are provided. The method permits the determination of 15 samples per h.
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(74)90266-6