Angular dependence of light scattering linewidths from hemoglobin solutions

We have measured the spectra of light scattered from solutions of oxy and carbon monoxy hemoglobin solutions under a variety of solution conditions, and as a function of scattering angle. In all cases we do not observe the anomalous positive intercepts in the plot of linewidth vs. the square of the scattering vector which have been reported by other workers and attributed to the kinetics of the dimertetramer interaction. We believe the anomalies may be attributable to the coincidence of the Helium-Neon wavelength and the 630 nm absorption of methemoglobin.