Clinical evaluation of routine measurement of liver and bone alkaline phosphatases in human serum: Differential inhibition by l-phenylalanine and carbamide (urea) on the LKB 8600 reaction rate analyzer

Determination of liver and bone alkaline phosphatase in human serum was carried out with a recently developed routine method using differential inhibition by l-phenylalanine and carbamide on the LKB 8600. Three groups of patients were investigated: 71 patients with hepato-biliary disorders, 22 patients with bone diseases, and 67 patients without recognizable hepato-biliary or bone diseases. Agreement between the clinical diagnosis and the separate values of liver AP and bone AP was found in patients with elevated total alkaline phosphatase activities in serum.