The role of tryptophan in neutral fructose diphosphatase

The role of tryptophan in neutral fructose diphosphatase

Neutral rabbit liver fructose-1,6-diphosphatase which does not contain tryptophan has been isolated and shown to possess catalytic properties identical to that for the neutral enzyme which contains tryptophan. Criteria include pH activity optimum, EDTA and oleate activation, AMP inhibition and speci...

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Veröffentlicht in:Biochemical and biophysical research communications 1974-03, Vol.57 (1), p.196-203
Hauptverfasser: Benkovic, S.J., Frey, W.A., Libby, C.B., Villafranca, J.J.
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine Monophosphate - pharmacology
Animals
Chromatography, Ion Exchange
Edetic Acid - pharmacology
Enzyme Activation
Fructose-Bisphosphatase - antagonists & inhibitors
Fructose-Bisphosphatase - metabolism
Hydrogen-Ion Concentration
Kinetics
Liver - enzymology
Oleic Acids - pharmacology
Rabbits
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Subtilisins
Time Factors
Tryptophan - analysis
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Beschreibung
Zusammenfassung:Neutral rabbit liver fructose-1,6-diphosphatase which does not contain tryptophan has been isolated and shown to possess catalytic properties identical to that for the neutral enzyme which contains tryptophan. Criteria include pH activity optimum, EDTA and oleate activation, AMP inhibition and specific activity. However, the enzyme lacking tryptophan responds differently to proteolysis by subtilisin exhibiting a more rapid loss of sensitivity to AMP inhibition.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(74)80376-1