use of ribonuclease U2 in RNA sequence determination. Some corrections in the catalog of oligomers produced by ribonuclease T1 digestion of Escherichia coli 16S ribosomal RNA

use of ribonuclease U2 in RNA sequence determination. Some corrections in the catalog of oligomers produced by ribonuclease T1 digestion of Escherichia coli 16S ribosomal RNA

The catalog of oligomers produced by ribonuclease T1 digestion of Escherichi coli 16S ribosomal RNA has been determined by a new method that involves the use of ribonuclease U2 from Ustilago sphaerogena. The sequences for the larger T1 oligomers (8 or more bases) determined in this way differ in mor...

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Veröffentlicht in:Journal of molecular evolution 1974-02, Vol.3 (1), p.63-77
Hauptverfasser: Uchida, T, Bonen, L, Schaup, H.W, Lewis, B.J, Zablen, L, Woese, C
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Base Sequence
Basidiomycota - enzymology
biochemical techniques
Cellulose
Chromatography, DEAE-Cellulose
Electrophoresis
Electrophoresis, Paper
enzyme activity
Escherichia coli
Escherichia coli - analysis
nucleotide sequences
nucleotides
oligonucleotide sequence analysis
oligonucleotides
Oligonucleotides - analysis
Pancreas - enzymology
Phosphorus Radioisotopes
Ribonucleases
Ribonucleotides - analysis
ribosomal RNA
RNA, Bacterial
RNA, Ribosomal
Ustilago
ustilago sphaerogena
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Zusammenfassung:The catalog of oligomers produced by ribonuclease T1 digestion of Escherichi coli 16S ribosomal RNA has been determined by a new method that involves the use of ribonuclease U2 from Ustilago sphaerogena. The sequences for the larger T1 oligomers (8 or more bases) determined in this way differ in more than 50% of the cases from those reported previously, (determined by other methods).
ISSN:0022-2844
1432-1432
DOI:10.1007/BF01795977