SAPONIFICATION AS A MEANS OF SELECTIVELY REVERSING THE METHYLATION BLOCKADE OF TISSUE BASOPHILIA
Saponification reverses the methylation blockade of cartilage and mast cell basophilia demonstrated by azure A above pH 3-4, but fails to do so at pH levels below this range. Saponification of oxidized hair cortex restores the methylation blocked basophilia demonstrable at pH 2 or higher. Mouse amyl...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 1959-03, Vol.7 (2), p.123-125 |
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| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
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| Online-Zugang: | Volltext |
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| Zusammenfassung: | Saponification reverses the methylation blockade of cartilage and mast cell basophilia demonstrated by azure A above pH 3-4, but fails to do so at pH levels below this range. Saponification of oxidized hair cortex restores the methylation blocked basophilia demonstrable at pH 2 or higher. Mouse amyloid is characterized by a weak basophilia which is relatively resistant to methylation.
The results are interpreted as indicating that methylation hydrolyzes the sulfate esters and esterifies other acid groupings in tissue sections. |
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| ISSN: | 0022-1554 1551-5044 |
| DOI: | 10.1177/7.2.123 |