Introduction of Specific Cleavages into RNAs of RNA Bacteriophages for Determination of Base Sequences

Large fragments of R17, MS2, and Qβ RNAs were obtained reproducibly by limited digestion by ribonuclease T1. After digestion of R17 and MS2 RNAs, two pairs of large fragments were obtained that probably resulted from specific cleavages of the whole molecules. The cleavages of MS2 RNA were produced a...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1974-03, Vol.71 (3), p.742-745
1. Verfasser:	Fuke, Motohiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetates Adenosine Triphosphate - metabolism Autoradiography Bacteriophages Base Sequence Biological Sciences: Cell Biology Coliphages - analysis Electrophoresis Electrophoresis, Polyacrylamide Gel Gels Genes Molecular Weight Molecules Nucleotide sequences Phosphorus Radioisotopes Phosphotransferases - metabolism Polynucleotides Polynucleotides - analysis Ribonucleases - antagonists & inhibitors Ribonucleases - metabolism Ribonucleotides - analysis RNA RNA Viruses - analysis RNA, Viral - analysis RNA, Viral - metabolism Species Specificity Sulfates - pharmacology Zinc - pharmacology
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Zusammenfassung:	Large fragments of R17, MS2, and Qβ RNAs were obtained reproducibly by limited digestion by ribonuclease T1. After digestion of R17 and MS2 RNAs, two pairs of large fragments were obtained that probably resulted from specific cleavages of the whole molecules. The cleavages of MS2 RNA were produced at points 36 and 47% from the 5′end.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.71.3.742