Propagation of Canine Distemper (CD) Virus in Tissue Culture

Summary A chick-embryo-adapted canine distemper virus was propagated successfully for 64 passages in cultures of total chick-embryo tissues. As measured on the chorioallantoic membrane of live chick embryos, average virus titers of these passages ranged between 103.0 and 104.5/ml infected culture su...

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Veröffentlicht in:	Experimental biology and medicine (Maywood, N.J.) N.J.), 1959-03, Vol.100 (3), p.551-554
Hauptverfasser:	Cabasso, V. J., Kiser, K., Stebbins, M. R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Distemper Virus, Canine Immunologic Tests Old Medline Reproduction Research Design Viruses - ethnology
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Summary A chick-embryo-adapted canine distemper virus was propagated successfully for 64 passages in cultures of total chick-embryo tissues. As measured on the chorioallantoic membrane of live chick embryos, average virus titers of these passages ranged between 103.0 and 104.5/ml infected culture suspension. These titers are of the same order as those obtained with live-chick-embryo-grown virus. Identity of tissue-culture-grown agent with original CD virus employed was established by virus neutralization method and by ferret immunization. Effective freeze-dried vaccines were produced with different passage levels of tissue-culture-grown virus.
ISSN:	0037-9727 1535-3702 1535-3699
DOI:	10.3181/00379727-100-24694