Extraction of RNA from mammalian tissue culture cells

Extraction of RNA from mammalian tissue culture cells

A rapid method is described for the effective fractionation and extraction of undergraded nuclear and cytoplasmic RNA species from mammalian tissue culture cells. It is compared in detail with several existing procedures.

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Bibliographische Detailangaben
Veröffentlicht in:Analytical biochemistry 1974-02, Vol.57 (2), p.403-412
Hauptverfasser: Atherton, K.T., Darby, G.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Fractionation
Cell Line
Cell Nucleus - analysis
Cricetinae
Culture Media
Cytoplasm - analysis
Electrophoresis, Polyacrylamide Gel
Evaluation Studies as Topic
HeLa Cells - analysis
HeLa Cells - cytology
Humans
Kidney - analysis
Kidney - cytology
Methods
Phosphates - metabolism
Phosphorus Radioisotopes
Rabbits
RNA - isolation & purification
RNA, Ribosomal - analysis
Thymidine - metabolism
Tritium
Uridine - metabolism
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Beschreibung
Zusammenfassung:A rapid method is described for the effective fractionation and extraction of undergraded nuclear and cytoplasmic RNA species from mammalian tissue culture cells. It is compared in detail with several existing procedures.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(74)90095-5