Temperature-dependence of Dinitrocresol Stimulation of Aerobic and Anaerobic Lactate Production in Ascites Tumor Cells

Summary Conversion of glucose to lactate by ascites tumor cells derived from the Ehrlich carcinoma, Crocker sarcoma 180, and Freund sarcoma is stimulated by 4,6-dinitro-o-cresol under both aerobic and anaerobic conditions. Degree of stimulation increases as temperature is lowered from 37° to 15°, although the absolute rate of lactate production diminishes. Aerobic lactate production is stimulated to a greater degree than anaerobic and the former exceeds the latter under certain conditions. The implications and possible mechanisms of these phenomena are discussed.