DNA-membrane association during the mitotic cycle of Physarum polycephalum

Association of DNA with the nuclear membrane was studied in the naturally synchronous slime mold Physarum polycephalum. Sucrose gradient analyses showed that DNA is bound to the membrane during all stages of interphase. The association is labile, but can be stabilized by spermine, which presumably a...

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Veröffentlicht in:	Experimental cell research 1973-01, Vol.79 (2), p.287-294
Hauptverfasser:	Oppenheim, Ariella, Wahrman, J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites Carbon Radioisotopes Cell Nucleus - analysis Cell Nucleus - metabolism Centrifugation, Density Gradient DNA - analysis DNA - biosynthesis DNA - isolation & purification DNA - metabolism DNA Replication Fungal Proteins - analysis Leucine - metabolism Mitosis Myxomycetes - metabolism Phosphates - metabolism Phospholipids - analysis Phosphorus Radioisotopes plant biochemistry plant physiology RNA - analysis Spermine - pharmacology Thymidine - metabolism Time Factors Tritium Uridine - metabolism
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Beschreibung
Zusammenfassung:	Association of DNA with the nuclear membrane was studied in the naturally synchronous slime mold Physarum polycephalum. Sucrose gradient analyses showed that DNA is bound to the membrane during all stages of interphase. The association is labile, but can be stabilized by spermine, which presumably acts on the membrane. Experiments with pulse-labeled DNA suggest that replication does not occur at a membranal site.
ISSN:	0014-4827 1090-2422
DOI:	10.1016/0014-4827(73)90447-3