Some aspects of sulfide oxidation by rat-liver preparations

1. 1. The sulfide-oxidizing system in rat liver does not require peroxide for its operation. 2. 2. The existence of a protein-bound intermediate, possibly a thiosulfonate, has been demonstrated. 3. 3. Sulfite may be involved as an intermediate in the in vitro formation of thiosulfate from sulfide. A reaction mechanism has been proposed which accounts for the accumulation of thiosulfate in in vitro systems. 4. 4. Some effects of inhibitors and chelators on the rat-liver system have been described. While 8-hydroxyquinoline stimulated the heat-labile fraction, EDTA stimulated the complete system through its action on the heat-stable fraction.