HETEROGENEITY OF CATALASE IN MORRIS HEPATOMAS AS REVEALED BY ISOELECTRIC FOCUSING AND DEAE-CELLULOSE COLUMN CHROMATOGRAPHY
The heterogeneity pattern of catalase in Morris hepatomas 7316A, 7800, 5123C, 7794A, 9618A, 16, and 9618B was examined by isoelectric focusing using Ampholine-carrier ampholyte and DEAE-cellulose column chromatography. Catalase in the liver of normal adult rats was fractionated by isoelectric focusi...
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Veröffentlicht in: | GANN Japanese Journal of Cancer Research 1973/12/31, Vol.64(6), pp.599-608 |
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Sprache: | eng |
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Zusammenfassung: | The heterogeneity pattern of catalase in Morris hepatomas 7316A, 7800, 5123C, 7794A, 9618A, 16, and 9618B was examined by isoelectric focusing using Ampholine-carrier ampholyte and DEAE-cellulose column chromatography. Catalase in the liver of normal adult rats was fractionated by isoelectric focusing into 5 major components each having its own isoelectric point at pH 6.95, 6.75, 6.50, 6.10, and 5.90. In all the hepatomas examined, the acidic components of catalase having isoelectric points at pH 6.10 and 5.90 were found to disappear, while basic components of the enzyme showed a definite appearance. Chromatographic analysis of the enzyme using DEAE-cellulose column showed that a considerable portion of the enzyme activity failed to be adsorbed on the ion exchanger, which may well support the above-mentioned appearance of the basic components of the enzyme. The isoelectric focusing pattern as well as the chromatographic elution characteristics of catalase in the hepatomas closely resembled those in the liver of fetal and newborn rats. It is suggested that the alteration in the heterogeneity of hepatomas may be ascribed to the dedifferentiating nature of cancer. |
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ISSN: | 0016-450X |
DOI: | 10.20772/cancersci1959.64.6_599 |