Chloroplast ribosomal RNA genes in the chloroplast DNA of Euglena gracilis
Euglena chloroplast DNA has a buoyant density in CsCI of 1.686. Shearing this DNA produces a satellite band at density 1.700. The satellite, easily lost during preparative CsCI gradient centrifugation of chloroplast DNA, contains the genes for chloroplast ribosomal RNA. Pure Euglena chloroplast DNA...
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| Veröffentlicht in: | Journal of molecular biology 1973-06, Vol.77 (1), p.125-132 |
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| container_title | Journal of molecular biology |
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| creator | Rawson, James R.Y. Haselkorn, Robert |
| description | Euglena chloroplast DNA has a buoyant density in CsCI of 1.686. Shearing this DNA produces a satellite band at density 1.700. The satellite, easily lost during preparative CsCI gradient centrifugation of chloroplast DNA, contains the genes for chloroplast ribosomal RNA. Pure
Euglena chloroplast DNA is shown to contain one set of ribosomal RNA genes for each 90 × 10
6 daltons of DNA. |
| doi_str_mv | 10.1016/0022-2836(73)90366-5 |
| format | Article |
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Euglena chloroplast DNA is shown to contain one set of ribosomal RNA genes for each 90 × 10
6 daltons of DNA.</description><subject>Animals</subject><subject>Centrifugation, Density Gradient</subject><subject>Chloroplasts - analysis</subject><subject>DNA - analysis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Euglena gracilis - analysis</subject><subject>Genes</subject><subject>Molecular Weight</subject><subject>Nucleic Acid Hybridization</subject><subject>Pressure</subject><subject>RNA, Ribosomal - isolation & purification</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Tritium</subject><subject>Ultrasonics</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVb_gcKeRA-ryeZjk4tQav2iKIieQ5qdbSO7m5rsCv57t7YUT8LAHN5nZpgHoVOCrwgm4hrjLEszScVFTi8VpkKkfA8NCZYqlYLKfTTcIYfoKMYPjDGnTA7QgGU9z-UQPU2WlQ9-VZnYJsHNffS1qZLX53GygAZi4pqkXUJi_2C3fejLZNotKmhMsgjGusrFY3RQmirCybaP0Pvd9G3ykM5e7h8n41lqKc_blHDFqclLaSwrOM_zzPSFpYS5EkJKmWFWEssYU6KkJdiMUWGVIAUoXmBLR-h8s3cV_GcHsdW1ixaqyjTgu6hlRhiWQvUg24A2-BgDlHoVXG3CtyZYrxXqtR-99qNzqn8Vat6PnW33d_Mait3Q1lmf32xy6J_8chB0tA4aC4ULYFtdePf_gR__Xn5s</recordid><startdate>19730615</startdate><enddate>19730615</enddate><creator>Rawson, James R.Y.</creator><creator>Haselkorn, Robert</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19730615</creationdate><title>Chloroplast ribosomal RNA genes in the chloroplast DNA of Euglena gracilis</title><author>Rawson, James R.Y. ; Haselkorn, Robert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-15953a7f8ac4d55772a72a088eb966888204f1c44496f3fec2436c961de95d0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Animals</topic><topic>Centrifugation, Density Gradient</topic><topic>Chloroplasts - analysis</topic><topic>DNA - analysis</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Euglena gracilis - analysis</topic><topic>Genes</topic><topic>Molecular Weight</topic><topic>Nucleic Acid Hybridization</topic><topic>Pressure</topic><topic>RNA, Ribosomal - isolation & purification</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Tritium</topic><topic>Ultrasonics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rawson, James R.Y.</creatorcontrib><creatorcontrib>Haselkorn, Robert</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rawson, James R.Y.</au><au>Haselkorn, Robert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chloroplast ribosomal RNA genes in the chloroplast DNA of Euglena gracilis</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1973-06-15</date><risdate>1973</risdate><volume>77</volume><issue>1</issue><spage>125</spage><epage>132</epage><pages>125-132</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>Euglena chloroplast DNA has a buoyant density in CsCI of 1.686. Shearing this DNA produces a satellite band at density 1.700. The satellite, easily lost during preparative CsCI gradient centrifugation of chloroplast DNA, contains the genes for chloroplast ribosomal RNA. Pure
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| fulltext | fulltext |
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| ispartof | Journal of molecular biology, 1973-06, Vol.77 (1), p.125-132 |
| issn | 0022-2836 1089-8638 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Centrifugation, Density Gradient Chloroplasts - analysis DNA - analysis Electrophoresis, Polyacrylamide Gel Euglena gracilis - analysis Genes Molecular Weight Nucleic Acid Hybridization Pressure RNA, Ribosomal - isolation & purification Spectrophotometry, Ultraviolet Tritium Ultrasonics |
| title | Chloroplast ribosomal RNA genes in the chloroplast DNA of Euglena gracilis |
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