Chloroplast ribosomal RNA genes in the chloroplast DNA of Euglena gracilis

Euglena chloroplast DNA has a buoyant density in CsCI of 1.686. Shearing this DNA produces a satellite band at density 1.700. The satellite, easily lost during preparative CsCI gradient centrifugation of chloroplast DNA, contains the genes for chloroplast ribosomal RNA. Pure Euglena chloroplast DNA...

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Veröffentlicht in:	Journal of molecular biology 1973-06, Vol.77 (1), p.125-132
Hauptverfasser:	Rawson, James R.Y., Haselkorn, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Centrifugation, Density Gradient Chloroplasts - analysis DNA - analysis Electrophoresis, Polyacrylamide Gel Euglena gracilis - analysis Genes Molecular Weight Nucleic Acid Hybridization Pressure RNA, Ribosomal - isolation & purification Spectrophotometry, Ultraviolet Tritium Ultrasonics
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Beschreibung
Zusammenfassung:	Euglena chloroplast DNA has a buoyant density in CsCI of 1.686. Shearing this DNA produces a satellite band at density 1.700. The satellite, easily lost during preparative CsCI gradient centrifugation of chloroplast DNA, contains the genes for chloroplast ribosomal RNA. Pure Euglena chloroplast DNA is shown to contain one set of ribosomal RNA genes for each 90 × 10 6 daltons of DNA.
ISSN:	0022-2836 1089-8638
DOI:	10.1016/0022-2836(73)90366-5