Orthophosphate analysis by the Fiske-SubbaRow method and interference by adenosine phosphates and pyrophosphate at variable acid pH

ATP, ADP, AMP, and pyrophosphate interfere with colour development in the Fiske-SubbaRow assay procedure. The extent of interference observed is dependent on the pH of the final assay system; small changes in pH at low pH values lead to large variations in the amount of ATP interference. Addition of...

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Veröffentlicht in:	Analytical biochemistry 1973-12, Vol.56 (2), p.566-570
Hauptverfasser:	Seddon, B., Fynn, G.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine Nucleotides Adenosine Adenosine Diphosphate Adenosine Monophosphate Adenosine Triphosphatases - analysis Adenosine Triphosphate Bacteria - enzymology Diphosphates Hydrogen-Ion Concentration Methods Molybdenum Osmolar Concentration Phosphates - analysis Quaternary Ammonium Compounds
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Beschreibung
Zusammenfassung:	ATP, ADP, AMP, and pyrophosphate interfere with colour development in the Fiske-SubbaRow assay procedure. The extent of interference observed is dependent on the pH of the final assay system; small changes in pH at low pH values lead to large variations in the amount of ATP interference. Addition of excess ammonium molybdate and neutralisation of any previous acid additions (such that the pH is maintained constant throughout all assay procedures) removes interference and P 1 can be estimated in the presence of relatively high ATP concentrations.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(73)90221-2