Gel filtration and preparative free isoelectric focusing of rabbit serum phosphorylphosphatase

A phosphorylphosphatase in rabbit serum, splitting the insecticide organophosphate paraoxon, can be isolated and partially purified by gel filtration. The isolated enzyme is separated into two fractions by Preparative Free Isoelectric Focusing. The isoelectric points of the two enzyme fractions are...

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Veröffentlicht in:	Life sciences (1973) 1973-08, Vol.13 (4), p.383-389
Hauptverfasser:	Zech, Ronald, Zürcher, Klaus
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cations, Divalent Chromatography, Gel Chromatography, Ion Exchange Drug Stability Evaluation Studies as Topic Hydrogen-Ion Concentration Isoelectric Focusing Isoenzymes - blood Isoenzymes - isolation & purification Kinetics Mathematics Methods Molecular Weight Paraoxon Phosphoric Monoester Hydrolases - blood Phosphoric Monoester Hydrolases - isolation & purification Rabbits
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Beschreibung
Zusammenfassung:	A phosphorylphosphatase in rabbit serum, splitting the insecticide organophosphate paraoxon, can be isolated and partially purified by gel filtration. The isolated enzyme is separated into two fractions by Preparative Free Isoelectric Focusing. The isoelectric points of the two enzyme fractions are 5.7 and 6.3. The molecular weight is about 500,000. The kinetic data of the enzyme are reported.
ISSN:	0024-3205 1879-0631
DOI:	10.1016/0024-3205(73)90230-0